Gamma 32P Atp. A preferred compound of the invention is [gamma32 P]adenosine5’0(3thiotriphosphate) hereinafter designated [gamma32 P]ATPgamma S and having the formula I ##STR2## The nucleoside thiotriphosphates of the invention can be prepared by enzymatic thiophosphorylation of a nucleoside diphosphate of the formula ##STR3## where A is defined above with a 32 P.
Atp And Gtp Hydrolysis Assays Tlc Sciencedirect from ScienceDirect.com
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Application of the [γ32P] ATP kinase assay to study
Incubation of [gamma32P]ATP with a molar excess of the membranebound form of mitochondrial ATPase (F1) results in binding of the bulk of the radioactive nucleotide in high affinity catalytic sites (Ka = 10(12) M1) Subsequent initiation of respiration by addition of succinate or NADH is accompanied by a profound decrease in the affinity for ATP.
Radioactive in vitro Kinase Assays Application Support
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Evidence of ectokinasemediated phosphorylation of
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Atp And Gtp Hydrolysis Assays Tlc Sciencedirect
Interaction of iron regulatory protein1 (IRP1) with ATP
(PDF) [gamma32P]ATP as a tracer of the fragmentation of
[gamma32P]ATP CAS#:2964070 Chemsrc
(gamma)[32 p](gamma) thioribonucleoside51
[108] Synthesis of γ32PATP ScienceDirect
The Kinetics of Rabbit Muscle Pyruvate Kinase. Initial
Characterization of an ATPase/dATPase activity …
CAS 2964070 [Gamma32p]atp Alfa Chemistry
ARP0102D250 Adenosine 5’triphosphate [gamma32P
ATP, [γ32P] 3000Ci/mmol 10mCi/ml Lead, 100 µCi …
[gamma32P]ATP C10H16N5O13P3 PubChem
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Gamma 32p Atp PerkinElmer Bioz
ATP from high affinity Energydependent dissociation of
Why is the Alpha position in 32P nucleotide used for
Using alpha[32P]dATP alpha[32P]ATP or gamma[32P]ATP only a single polypeptide (Mr approximately 174000) was photolabeled in a manner completely consistent with the enzymology of ATP and dATP hydrolysis cell fractionation studies revealed a predominantly or exclusively nuclear localization.
